Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: Screening of 19 dyed nylon affinity networks for their ability to capture E. coli spiked in PBS. The y -axis represents the percentage of bacteria depleted by each dyed nylon material calculated based on the starting bacterial loads. The error bars represent the standard deviation calculated from at least two independent experiments run with duplicate plates.

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Bacteria, Standard Deviation

Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: Scanning electron microscopy demonstrates that incorporation of Alcian Blue into the nylon matrix enables the capture of E. coli from suspension. Top row: unprocessed nylon imaged before E. coli exposure shows smooth fiber surfaces. Second row: unprocessed nylon incubated with E. coli in PBS shows no evidence of bacterial binding. Third row: processed nylon (exposed to the dye bath without dye) displays increased surface irregularities but no bacterial adhesion. Bottom two rows: Alcian Blue nylon incubated with E. coli shows significant bacterial capture and adhesion to the nylon.

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Electron Microscopy, Suspension, Incubation, Binding Assay

Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: Evaluation of depletion of E. coli and S. epidermidis in PBS and 1:1 human plasma at two concentrations using Alcian Blue affinity networks. Binding was conducted in 1 mL suspensions and 0.5 mL was plated in duplicate on agar. The error bars represent the standard deviation calculated from at least two independent experiments run on duplicate plates. S. epi ., S. epidermidis .

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Clinical Proteomics, Binding Assay, Standard Deviation

Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: Schematic of sequential batch incubation of 50 mL bacterial suspensions (2 cfu/mL) with three nylon affinity networks (90 mg each). After each exposure, two 5 mL aliquots were transferred to bacterial growth media (LB for E. coli or TSB for S. epidermidis ), and the nylon sheet was plated on agar. The procedure was repeated for three sequential incubations.

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Incubation

Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: ( A ) Optical density at 600 nm of E. coli recovered in the unbound fractions across three sequential exposure steps to nylon affinity networks grown in LB media. ( B ) OD 600 measurements of S. epidermidis recovered in the unbound fractions as in ( A ). ( C ) Affinity networks placed on agar plates after exposure to E. coli in three sequential steps: nylon 1 (left), nylon 2 (center), nylon 3 (right) and control unexposed nylon (far right). The figures’ closeups are nylon 1 and unexposed nylon. The size of each nylon affinity networks was approximately 3.2 cm × 4.5 cm. The error bars represent the standard deviation calculated from at least two independent experiments run on duplicate plates.

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Control, Standard Deviation

Journal: Pathogens

Article Title: Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma

doi: 10.3390/pathogens14080778

Figure Lengend Snippet: Optical density at 600 nm of bacterial growth in the unbound fractions collected after each of three sequential filtrations using Alcian Blue nylon affinity networks. ( A ) E. coli spiked in PBS. ( B ) S. epidermidis spiked in PBS. ( C ) E. coli spiked in human plasma. ( D ) S. epidermidis spiked in human plasma. The error bars represent the standard deviation calculated from at least two independent experiments run on duplicate plates.

Article Snippet: Escherichia coli ( https://www.atcc.org/products/25922 (accessed on 30 July 2025)) was cultured in Luria–Bertani (LB) broth and Staphylococcus epidermidis ( https://www.atcc.org/products/12228 (accessed on 30 July 2025)) was cultured in Tryptic Soy broth (TSB) at 37 °C to the logarithmic phase (we used 1 OD 600 nm equivalent to 8 × 10 8 cells/mL for all our stock bacterial estimates) and diluted to approximately 10 cfu/mL and 100 cfu/mL in PBS or a 1:1 mixture of human plasma (Innovative Research, Inc., Novi, MI, USA) in PBS.

Techniques: Clinical Proteomics, Standard Deviation